| Methodologies |
| Feeding Tactics/Medium: |
20 drops F/2 (Micro Algae Grow from Florida Aqua Farms) added to each 2l bottle after split
|
| Split Cycle: |
Roughly every 7 or 8 days. Sometimes they've gone over a month between splits |
| Split Yield: |
I take 3/4 of the bottle and start a new culture with the remaining 1/4 |
| Number of Cultures: |
8 two liter bottles |
| Number of Splits: |
every 7 or 8 days (or as needed) |
| Density: |
15 million cells per ml (roughly)
|
| Harvest Methology: |
Pour 3/4 of the bottle into a spare 2 liter bottle to be used. Take the remaining 1/4 bottle and refill with 1.019 specific gravity Instant Ocean water. Add 20 drops of Guillard's F/2 and place the bottle back on the shelf |
| Adjustments Made: |
None. Once nannochloropsis is established, it is very forgiving. |