| Methodologies |
| Feeding Tactics/Medium: |
15-20 drops of F/2 into 2L of salt water, and then use the mixed salt water as the new medium to fill up the culture bottle.
|
| Split Cycle: |
Planning to split every 9 or 10 days. First split was on the 13th day due to mis-counted the days. |
| Split Yield: |
3/4 of the bottle culture (1500ml) is removed for feeding and for new culture. |
| Number of Cultures: |
Three 2L bottles |
| Number of Splits: |
One |
| Density: |
Unknown. The color is medium brown.
|
| Harvest Methology: |
Pour out 1/2 of the bottle for feeding. Pour another 500ml into a new bottle for splitting to another culture.
When I split and create a new culture, I only use new bottles for the new culture. For the original dense culture bottle, after I pour out the amount that I use, I just refill it with salt water. The original bottle will be keep re-using until there are stuff or film developing near the bottle neck, then I just trash the bottle (by putting them into the city recycle bin. Not really trashing it). I do not clean or sterilize any them. |
| Adjustments Made: |
None. |