| Methodologies |
| Feeding Tactics/Medium: |
NSW prepared in 1 gallon plastic container (old milk jug) sterilized by adding 1ml chlorox bleach per gallon. 1 quart culture bottle filled 2/3 to 3/4 with sterile medium, 20 drops Guillard's f/2 added, and dechlorinated with 0.4ml Drs. Foster & Smith Chlorine Neutralizer ( 15 minutes before inoculation).
|
| Split Cycle: |
Cultures maturing for split abut every 7-10 days |
| Split Yield: |
Two-thirds to 3/4 of quart bottle |
| Number of Cultures: |
3 at present |
| Number of Splits: |
Splitting a bottle every 2-3 days with my current cultures |
| Density: |
Not measured
|
| Harvest Methology: |
2/3 to 3/4 of mature bottle poured off for feeding, remainder used to inoculate new culture bottle. |
| Adjustments Made: |
None so far |