MBI Event Reporting Form

GENERAL
Name:	Zach Mueller ( Amphispur ) Site: The Southern Colorado Marine Aquarists Society
Species:	Dunaliella tertiolecta ( Class: FC2 , Points: 10 )
Establishment Date:	03/08/2014
Culture Source:	http://florida-aqua-farms.com/shop/microalgae-disks/
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=88749

Culturing Vessel Details
Temperature:	Ambient, around 75-78 ° F
PH:	Not Measured
Specific Gravity:	1.028
Vessel Description:	500ml canning jar with a plastic lid, and an aeration rate of around 5-6 bubbles per second
Lighting:	3 Lights of America Linkable Fluorescent Undercabinet Portable Lights
Lighting Schedule:	22 Hours on, 2 hours off

Methodologies
Feeding Tactics/Medium:	10 Drops of Guillards F/2 formula
Split Cycle:	Every 2-3 days
Split Yield:	~25-50% per culture
Number of Cultures:	2
Number of Splits:	At least 20
Density:	10,300,000 cells/ml
Harvest Methology:	1. Add 2x Guillards F/2 formula per 100ml of culture water 2. Heat in microwave until 212F has been achieved 3. Allow cooling until room temperature 4. Split algae into containers 5. Run airline into culture with a mild-turbulent flow 6. Have warm lights on the culture running at 22 hours on, 2 hours off 7. Split every day or two, or until the jar has a light green tint to it 8. Repeat 2-6 for every split
Adjustments Made:	None

Other Details
Other Information: