| Methodologies |
| Feeding Tactics/Medium: |
A squirt of Iso or RG complete for feeding as needed, and split using ~1.022 (ish) ASW that has been sterilized (1/2mL bleach per liter for 1 hours, neutralized with 1.5mL of 1M sodium thiosulfate per liter)
|
| Split Cycle: |
Every few days, as the culture appears to need it |
| Split Yield: |
Not measured. Since this is a newly established culture, it is not very dense |
| Number of Cultures: |
2 |
| Number of Splits: |
~5 |
| Density: |
Not measured, but the population has been steadily increasing
|
| Harvest Methology: |
To "harvest," part of the culture is simply dumped into wherever I want them to go. No sieving. |
| Adjustments Made: |
None as of yet, other than adding aeration once the culture was in enough water to allow it. |