MBI Event Reporting Form

GENERAL
Name:	Ken Rood ( Lrood ) Site: Marine Breeding Initiative (MBI)
Species:	Isochrysis galbana ( Class: FC2 , Points: 10 )
Establishment Date:	03/12/2014
Culture Source:	Neptunes Garden
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=89777

Culturing Vessel Details
Temperature:	70 ° F
PH:	not directly measured, but typically 8.0-8.1
Specific Gravity:	1.020
Vessel Description:	1 quart plastic bottles (Gatorade bottles), with plans to expand to 2 liter plastic soda bottles as cultures grow
Lighting:	13w spiral cfl bulb
Lighting Schedule:	16 hours light, 8 hours dark

Methodologies
Feeding Tactics/Medium:	New SW (Reef Crystals or Instant Ocean) prepared at 1.020. I am trying both microwave (to temp of 180-190 degrees F), and bleach sterilization (standard household bleach at 1 ml/gallon SW prepared usually about 1 week in advance, neutralized with AmQuel immediately prior to use) processes. Approx 12 drops Guillard's f/2 add to each liter of culture medium
Split Cycle:	pending, hopefully every 7-10 days
Split Yield:	pending
Number of Cultures:	2
Number of Splits:	pending
Density:	unknown
Harvest Methology:	Mature cultures will be used for copepod feeding, and brown water in larval tanks. Approximately 1/3 of each mature culture will be used to establish new cultures.
Adjustments Made:	pending

Other Details
Other Information:	New phyto culture rack is the works with 2 x 40w fluorescent bulbs.