MBI Event Reporting Form

GENERAL
Name:	Shannon Pechauer ( shannpeach ) Site: Marine Breeding Initiative (MBI)
Species:	Brachionus rotundiformis ( Class: FC1 , Points: 5 )
Establishment Date:	09/05/2013
Culture Source:	http://www.seahorsesource.com/cgi-bin/shop/detail.cgi?id=201140
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=86282

Culturing Vessel Details
Temperature:	Room temperature, ~70-80 ° F
PH:	Not measured
Specific Gravity:	~1.022
Vessel Description:	One gallon clear plastic container
Lighting:	Ambient light from southern facing window and a two-bulb fluorescent strip light
Lighting Schedule:	6 AM to 9PM

Methodologies
Feeding Tactics/Medium:	Culture is fed live phyto (Nannochloropsis, Tetraselmis, Isochrysis, Rhodomonas, or Chaetoceros) or roti-rich (Florida Aqua Farms) once or twice daily (twice daily if the rotifer density is high) enough to tint the water slightly
Split Cycle:	Once every 3 to 7 days
Split Yield:	Not counted/measured
Number of Cultures:	One
Number of Splits:	Not counted
Density:	Not counted; culture gets more dense when fed live phyto
Harvest Methology:	Approximately once or twice a week 75-90% of the culture is dumped out and new, sterilized ASW is added. The entire culture is moved to a new/sterilized container once every two or three weeks
Adjustments Made:	None

Other Details
Other Information: