| Methodologies |
| Feeding Tactics/Medium: |
1/2mL per liter of Florida Aqua Farm's Microalgae Grow in sterilized water (1mL of bleach per L to sterilize for at least an hour, following by 1.5mL 1M sodium thiosulfate per L to neutralize)
|
| Split Cycle: |
Currently splitting every three days to scale up and make more culture bottles. Culture may be able to go longer without being split, but at this point I don't have enough backups to do a culture-crash test to see how long it can go between splits. |
| Split Yield: |
Each split is made to increase the total volume so typically each split at least doubles the previous amount. This is anywhere from 400mL to 2000mL at this point. |
| Number of Cultures: |
2 currently, goal is to have at least 3 |
| Number of Splits: |
3 so far to scale up |
| Density: |
Not measured
|
| Harvest Methology: |
I take half of the culture and double or triple the volume using fertilized, sterilized ASW. Currently, splits have been focused on scaling up more so than "harvesting" to use for anything. |
| Adjustments Made: |
None |