MBI Event Reporting Form

GENERAL
Name:	Shannon Pechauer ( shannpeach ) Site: Marine Breeding Initiative (MBI)
Species:	Isochrysis galbana ( Class: FC2 , Points: 10 )
Establishment Date:	04/18/2013
Culture Source:	Fellow Hobbyist
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=82278

Culturing Vessel Details
Temperature:	Ambient room temp, ~72 ° F
PH:	Not measured
Specific Gravity:	1.020
Vessel Description:	2L soda bottles
Lighting:	Ambient light from southern facing window supplemented with 2-bulb fluorescent strip light as needed
Lighting Schedule:	Sunrise to sunset, supplemented 6PM to 9PM

Methodologies
Feeding Tactics/Medium:	1/2mL of Florida Aqua Farm's Microalgae grow per 1L of sterilized water.
Split Cycle:	Approximately every 7 days, or as needed
Split Yield:	Not measured
Number of Cultures:	2
Number of Splits:	Not counted
Density:	Not measured
Harvest Methology:	Once culture is dark enough (I don't wait until it is "chocolate brown," I split when it is more of a dark "gold" color) I harvest 2/3 of the bottle and replace with sterilized, fertilized broodstock tank water
Adjustments Made:	None

Other Details
Other Information:	Water is sterilized using bleach (1/2mL per L of concentrated bleach) and neutralized using sodium thiosulfate (1.5 mL per L of 1M sodium thiosulfate).