MBI Event Reporting Form

GENERAL
Name:	Shannon Pechauer ( shannpeach ) Site: Marine Breeding Initiative (MBI)
Species:	Tetraselmis chuii ( Class: FC1 , Points: 5 )
Establishment Date:	04/16/2013
Culture Source:	Florida Aqua Farms MicroAlgae Disk
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=82191

Culturing Vessel Details
Temperature:	~72 ° F
PH:	Not measured
Specific Gravity:	1.020
Vessel Description:	2L soda bottles
Lighting:	Ambient lighting from southern facing window, supplemented with a 2-bulb compact fluorescent strip light
Lighting Schedule:	Sunrise to sunset. The strip light is turned on from 6PM to 9PM on shorter days

Methodologies
Feeding Tactics/Medium:	1/2mL FAF MicroAlgae Grow per 1L of sterilized water
Split Cycle:	Every 4 to 10 days depending on how dense the culture is
Split Yield:	Unknown
Number of Cultures:	3
Number of Splits:	Numerous
Density:	Not measured
Harvest Methology:	2/3 of the 2L bottle is taken and is then replaced with sterilized, fertilized broodstock water. Excess is stored in the refrigeratorand mixed daily until needed.
Adjustments Made:	None

Other Details
Other Information:	Sterilization is done using 1/2mL of bleach per L of water and allowed to sit for minimum one hour. 1.5 mL sodium thiosulfate per L of water is used to nuetralize (stock sodium thiosulfate is 1.1M).