| Methodologies |
| Feeding Tactics/Medium: |
70% T-Iso and 30% T. Chuii from http://www.mbisite.org/Forums/tm.aspx?m=69565 and http://www.mbisite.org/Forums/tm.aspx?m=69563
Food was maintained at a mid green tint with 2-3ml added every three days or as required for the 2000ml flasks. The goal was to just be able to see the airline on the far side of the flask at the bottom.
Larger buckets (10L water) had food added until the bottom of the bucket was not visible through the water. This was adjusted every few days. The amount used varied significantly (eg 5ml to 100ml) depending on the currently volume of copepods in the bucket.
|
| Split Cycle: |
Cultures are split once the density of the copepods on the glass looks to be crowded.
The two master flasks are maintained, excess is stored in 2 x 20L salt buckets |
| Split Yield: |
flask is split in two, one half remains for master culture and is topped up with new water and food. Other half is used as food, these are drained via a 50um sieve and then back flushed into the two salt buckets. |
| Number of Cultures: |
3 initially, 4 later |
| Number of Splits: |
Not counted |
| Density: |
Not meastured
|
| Harvest Methology: |
approx once a month alternate master flasks are 50% drained and the copepods moved to buckets. The other flask is drained the following month.
After the first 2 months, this process is performed on an ongoing basis until Dec 2012 when the culture perished due to summer heat. |
| Adjustments Made: |
Started with large flat 10L container, this was shutdown.
Added 2 x 20L salt buckets 1/2 filled with salt water as storage and additional growth room.
Topup for evaperation was new artificial salt water. |