Culture Continuation Report Form

GENERAL
Name:	Daniel Walden ( waldend ) Site: Marine Breeding Initiative (MBI)
	Tisbe biminiensis ( Class: FC1 , Points: 5 )
Establishment Date:	11/08/2014
Continuation Date:	02/08/2015
Culture Source: (link if possible)	Algagen
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=93611

Culturing Vessel Details
Temperature:	Ambient Room Temp (70°F-78°F in summer, 60°F-70°F
pH:	Not Tested
Specific Gravity:	1.024
>Vessel Description:	~ 1 gallon plastic square footprint container 2x height to width ratio with large diameter screw on lid.
Lighting:	Ambient room lighting with slight illumination from nearby phyto cultures
Lighting Schedule:	24 hour ambient (light fixture for phyto in room at night)

Methodologies
Feeding Tactics/Medium:	I alternate between feeding Tet and ground up flake food. Typically feed every 2-3 days. Tet is enough to lightly tint the water. Flake food is probably .5 square inches of flake.
Split Cycle:	I split these when the population seems large and not noticeably increasing. Guestimate of 2000+ copepods.
Split Yield:	For ramp up I poured 50% into new culture and refilled both with sterilized ASW.
Number of Cultures:	3
Number of Splits:<	5
Density:	Not counted
Harvest Methodology:	Sieve through 23micron filter.
Adjustments Made:	None so far

Other Details
Other Information: