Culture Continuation Report Form

GENERAL
Name:	Mindy van Leur ( EasterEggs ) Site: Marine Breeding Initiative (MBI)
	Tigriopus californicus ( Class: FC1 , Points: 5 )
Establishment Date:	04/02/2014
Continuation Date:	10/09/2014
Culture Source: (link if possible)	Reed Mariculture
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=70268

Culturing Vessel Details
Temperature:	Ambient room temperature 76-80 ° F
pH:	Not tested
Specific Gravity:	1.025 to 1.030
>Vessel Description:	Mostly 15x15x10" clear plastic Sterilite storage containers.
Lighting:	Ambient room light from overhead fluorescent.
Lighting Schedule:	14 hours light, 10 hours dark.

Methodologies
Feeding Tactics/Medium:	RotiGrow Plus fed when culture becomes colorless, and ClorAm-X solution used at same volume - about 1 mL per gallon of culture.
Split Cycle:	None.
Split Yield:	None.
Number of Cultures:	5
Number of Splits:<	0
Density:	Unknown. Culture waxes and wanes.
Harvest Methodology:	Siphon part of the culture through a 180 micron sieve nesting in a 53 micron sieve. collect the ones off the 180 micron sieve and put back what is caught on the 53 micron sieve.
Adjustments Made:	I painted the sides of the containers. The copepods do not like clear-sided containers.

Other Details
Other Information:	It would be nice to know what causes the waxing and waning of the density.

Umm_fish?
2/4/2015 2:35:45 PM

Mindy, would you mind putting up another picture or video to confirm the continuation of the culture? Thanks!

EasterEggs
2/5/15
11:6:33 PM

Hi Andrew. Do you see the photo from Oct? This shows continuation from April to Oct. I took a crappy cell phone pic a couple days...I can post that.

Umm_fish?
2/6/15
9:20:25 AM

I see them now. Thanks!