| Methodologies |
| Feeding Tactics/Medium: |
NSW prepared (typically Instant Ocean or Reef Crystals) at sg of 1.020 in old 1 gallon plastic milk jugs. Solution is sterilized using 1ml/gallon NSW of standard household bleach. When ready for use, I fill a culture bottle 2/3-3/4 full with the sterile culture medium, at 0.4ml of Drs Foster & Smith Chlorine Neutralizer and 20 drops Guillards f/2 (dechlor done at least 15 minutes before innoculation). Bottle then innoculated from a mature bottle, leaving about 2" unfilled space (to avoid foaming with aeration). Aeration is via rigid tubing through the drilled bottle cap.
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| Split Cycle: |
Cultures mature in about 8-9 days, and are then used to feed tanks & innoculate new culture bottles.
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| Split Yield: |
About 500ml available for feeding from each bottle, not including the amount to start a new culture.
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| Number of Cultures: |
Currently maintaining 3 active bottles
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| Number of Splits:< |
numerous
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| Density: |
not measured
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| Harvest Methodology: |
When a bottle is mature (dark golden brown to the eye), one-fourth to one-third is poured into a freshly prepared bottle for a new culture. The remainder is used as food.
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| Adjustments Made: |
When I first tried t-iso, I used AmQuel as my dechlorinating agent. I didn't realize that this product also neutralized ammonia (thanks to Kathy for pointing this out). This initial attempt slowly dwindled and failed. With the most recent restart, I have been using Drs Foster & Smith Chlorine Neutralizer with great results.
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