Culture Continuation Report Form

GENERAL
Name:	Zach Mueller ( Amphispur ) Site: The Southern Colorado Marine Aquarists Society
	Isochrysis galbana ( Class: FC2 , Points: 30 )
Establishment Date:	12/03/2012
Continuation Date:	07/23/2013
Culture Source: (link if possible)	Florida Aqua Farms Inc.
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=78352&high=

Culturing Vessel Details
Temperature:	Ambient, Around 70 ° F
pH:	Not Measured
Specific Gravity:	1.023
>Vessel Description:	One quart wide-mouthed jar with a plastic lid and airline going into the culture
Lighting:	6500k wat leds that are about 10 cm away from the culture
Lighting Schedule:	14 hours on, 10 hours off

Methodologies
Feeding Tactics/Medium:	FAF Micro Algae Grow of 10 drops per quart at the beginning of each culture
Split Cycle:	Every 8 Days
Split Yield:	1/2 of original
Number of Cultures:	4
Number of Splits:<	29
Density:	~7.2 million per ml, ~ 6000000000 cells
Harvest Methodology:	1/4 of a quart out of mother culture and moved into separate prepared container that was premade with fresh saltwater and FAF microalgae grow in the microwave for 42 minutes for 7 jars
Adjustments Made:	None

Other Details
Other Information:

Thread

Umm_fish?
8/1/2013 8:42:44 PM

Any possibility that you could take a microscope picture of those cultures? Those don't really look like healthy Iso cultures to me and I'm not sure I've ever heard of any that have lasted that long. Thanks!

Amphispur
8/3/13
11:26:26 PM

Of Course! Sorry it took me so long to respond, company, sports etc, but I got a microscope evidence of it. It is alive, and thriving.

Umm_fish?
8/6/13
1:53:7 PM

Looks good to me. Congratulations!

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