| Methodologies |
| Feeding Tactics/Medium: |
Rotifers fed about 2.25 mL RotiGrow Plus per liter of culture water split into 2 daily feedings. Water is still barely green at the next feeding. A ClorAm-X solution is made using 4 tsp ClorAm-X and enough water to make 250 mL of solution. The ClorAm-X solution is used in equal amounts of RG+ at each feeding.
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| Split Cycle: |
Day 1 - decant top 1/6 clean culture, dump rest of culture, gently clean bucket (no disinfectants), return 1/6 clean culture, add fresh saltwater to make 1/3 bucket volume. Day 2 - add 1/3 bucket volume to make 2/3 total volume. Day 3 - same as Day 2 to make 3/3 total volume. Day 4 - repeat starting at "Day 1".
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| Split Yield: |
Just under 3L fairly dense rotifers.
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| Number of Cultures: |
2
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| Number of Splits:< |
Continuous
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| Density: |
Not counted.
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| Harvest Methodology: |
Harvest as needed; about 500 mL per feeding, 2-3 feedings per week.
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| Adjustments Made: |
I was doing 30% (pseudo)harvest daily, but found I could not keep high densities and the cultures became too time consuming. I started the split cycle described above in May 2012 and haven't had any crashes. I find it easier to maintain.
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