Culture Continuation Report Form

GENERAL
Name:	Darren Nancarrow ( CaptCrash ) Site: Marine Breeding Initiative (MBI)
	Isochrysis galbana ( Class: FC2 , Points: 30 )
Establishment Date:	03/27/2012
Continuation Date:	06/25/2012
Culture Source: (link if possible)	http://www.marine.csiro.au/algaedb//FMPro?-db=cmarc%20database.fp5&-format=record_detail.htm&-lay=everything&-sortfield=genera%20plus%20species&-op=eq&Culturestatus=Published&-op=eq&CS%20Number=CS-177&-max=15&-recid=35112&-find=
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=69565

Culturing Vessel Details
Temperature:	Not measured (Fans turn on at 20c to cool algae cu
pH:	Not measured
Specific Gravity:	1.015
>Vessel Description:	2000ml Erlenmeyer Flask/Conical Flask and plastic soda bottles
Lighting:	CF Light, 3 x double bulb, 37W fixtures. 4 x 5000k bulbs installed (2 spares)
Lighting Schedule:	16 hours per day, 7am to 11pm

Methodologies
Feeding Tactics/Medium:	RO/DI water with Artificial Salt to 1.015. Micro Algae Grow®, Florida Aqua Farms Inc added at a rate of 0.4ml / 1000ml salt water. Media is microwaved in an 1100W microwave for 17.5 minutes per 2.5L batch. Batch is allowed to cool to room temperature before use.
Split Cycle:	Cultures are split, 500ml to two or three new soda bottles, 500ml to a new clean flask. Remainder to fridge to feed as required. Split depends on dark colour/density of culture.
Split Yield:	1:4 every 10-12 days.
Number of Cultures:	3
Number of Splits:<	more than 10 resulting 2000ml samples
Density:	not measured
Harvest Methodology:	2000ml soda bottles are used to feed copepods and SS rotifers. Either of the following is used 1. Approx 1500ml is used from each bottle and the remaining 500ml is topped up with new media 2. the entirety of the bottle is used to feed and a new 500ml starter added from master cultures.
Adjustments Made:	As evaperation occurs within the containers, they are topped up with additional microwaved media

Other Details
Other Information: