| Methodologies |
| Feeding Tactics/Medium: |
RO/DI water with Artificial Salt to 1.015. Micro Algae Grow®, Florida Aqua Farms Inc added at a rate of 0.4ml / 1000ml salt water.
Media is microwaved in an 1100W microwave for 17.5 minutes per 2.5L batch. Batch is allowed to cool to room temperature before use.
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| Split Cycle: |
Cultures are split, 500ml to two or three new soda bottles, 500ml to a new clean flask. Split depends on dark colour/density of culture.
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| Split Yield: |
1:3 or 1:4 every 7-10 days.
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| Number of Cultures: |
3
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| Number of Splits:< |
more than 20 resulting 2000ml samples
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| Density: |
not measured
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| Harvest Methodology: |
2000ml soda bottles are used to feed rotifers, artimia, copepods and to provide green water for new fry RBT's.
Either of the following is used
1. Approx 1500ml is used from each bottle and the remaining 500ml is topped up with new media
2. the entirety of the bottle is used to feed and a new 500ml starter added from master cultures.
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| Adjustments Made: |
As evaperation occurs within the containers, they are topped up with additional microwaved media
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