Culture Continuation Report Form

GENERAL
Name:	John Berns ( patent ) Site: Twin Cities Marine Aquarium Society
	Brachionus plicatilis ( Class: FC1 , Points: 5 )
Establishment Date:	10/31/2011
Continuation Date:	04/12/2012
Culture Source: (link if possible)	KBB0118 (local breeder)
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=65056&high=

Culturing Vessel Details
Temperature:	Ambient, about 72-75 ° F
pH:	unmeasured
Specific Gravity:	1.020
>Vessel Description:	5 gallon bucket
Lighting:	single fluorescent tube overhead. Lighting is mainly incidental, its not directly under lights.
Lighting Schedule:	14 hours on

Methodologies
Feeding Tactics/Medium:	Add either Nanno 3600 or live nanno culture twice daily, 12 hours apart (morning and evening), additions dependent on previous addition being consumed.
Split Cycle:	Split off 1/3 daily through 12/13 and replace with fresh saltwater. Doing water changes monthly. Stir it before splitting.
Split Yield:	unknown
Number of Cultures:	1
Number of Splits:<	daily
Density:	unknown
Harvest Methodology:	53 micron sieve, dump water.
Adjustments Made:	started doing a monthly complete water change

Other Details
Other Information:

Thread

Umm_fish?
4/13/2012 9:41:18 AM

John--Just a reminder about the photos I asked for in the other form. Thanks!

patent
4/13/12
4:12:53 PM

I uploaded 2, one with flashlight underneath, but had trouble getting a good photo that way, and one with flashlight to the side.

Umm_fish?
4/14/12
10:20:3 AM

Thanks!

KathyL
5/18/2012 9:04:02 PM

Hi John, The continuation date in the report has to match the continuation date in the journal, and it has to be 90 days or more after the establishment date. Sorry to nit pick, but it's required for approval.

patent
5/20/12
6:0:9 PM

Fixed it.

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Thread