Culture Continuation Report Form

GENERAL
Name:	Amanda Cox ( AmandaCox ) Site: Marine Breeding Initiative (MBI)
	Brachionus plicatilis ( Class: FC1 , Points: 5 )
Establishment Date:	09/18/2011
Continuation Date:	12/18/2011
Culture Source: (link if possible)	Mike Taylor, CORA Frag Swap, his original was from livebrineshrimp.com
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?m=60645

Culturing Vessel Details
Temperature:	74 ° F
pH:	8.3
Specific Gravity:	1.015
>Vessel Description:	Two five gallon buckets, one with an airstone, one with an airline
Lighting:	Ambient fluorescent shop lights
Lighting Schedule:	8 hrs / day on

Methodologies
Feeding Tactics/Medium:	Currently feed RotiGrow+
Split Cycle:	Split 11/6 by removing 64 oz. and placing into new clean bucket
Split Yield:	New culture is ~15/ml
Number of Cultures:	2
Number of Splits:<	1
Density:	Original >50/ml, new ~15/ml
Harvest Methodology:	About every 2-3 days, harvest using 120um sieve and then 53um sieve.
Adjustments Made:	See journal

Other Details
Other Information:

Thread

fishtal
1/28/2012 9:28:25 PM

Can you post a few micro pics so we can be sure they are actually rotifers? Thank

AmandaCox
2/17/12
3:17:35 PM

Well, so glad you asked...lol I bought a Celestron 44340 last week and got to use it today. I could not find any female rotifers in the culture. I think I might have found one male and took a picture, but it's a stretch. There was quite a bit of algae. I'll be starting over soon!

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