Culture Continuation Report Form

GENERAL
Name:	Glen King ( rgrking ) Site: Marine Aquarium Societies of North America
	Nannochloropsis Sp. ( Class: FC1 , Points: 5 )
Establishment Date:	04/30/2011
Continuation Date:	08/25/2011
Culture Source: (link if possible)	http://florida-aqua-farms.com/secure/agora.cgi
Link to Journal:	http://www.mbisite.org/Forums/tm.aspx?high=&m=52937&mpage=1#52937

Culturing Vessel Details
Temperature:	72 ° F
pH:	8.0
Specific Gravity:	1.022
>Vessel Description:	2 liter soda bottle
Lighting:	ambient lighting and 24" flouresent 6700k bulb
Lighting Schedule:	on all the time.

Methodologies
Feeding Tactics/Medium:	Dose micro algae food 0.5ml each day
Split Cycle:	split as needed when culture is dark enough. usually around 1-2 weeks.
Split Yield:	1L diluted with .5L in new bottle
Number of Cultures:	3
Number of Splits:<	6
Density:	unknown
Harvest Methodology:	only split when dark enough not to see through. I add just about a cup of water to each after I split it, and wait for it to darken again before I add more water.
Adjustments Made:	I have a very fast rolling bubble now. I've found it helps to keep it alive. I lost a couple of bottles that were not getting a rolling bubble

Other Details
Other Information:	I think RG+ would be easier and may go to it later on.

Thread

fishtal
8/27/2011 4:29:07 PM

We need your split methodology in your journal too.

rgrking
8/27/11
4:36:0 PM

Thanks Tal, It should be all completed now.

JimWelsh
9/5/2011 11:42:57 AM

1) Need continuation date in the journal. 2) Need the amount of FAF Micro Algae Grow used in the journal. 3) Need the methods you use to prepare (sterilize) the media and containers, if any. 4) In the report you say, "Dose micro algae food 10ml each day" -- do you really dose 10 ml of FAF Micro Algae Grow in each 2 L bottle each day? That sounds like an awful lot to me!

rgrking
9/5/11
12:15:15 PM

working on it now. It's not mL it should be cc. sorry about that.

rgrking
9/5/2011 12:26:11 PM

Ok Jim, Should be good to go.

JimWelsh
9/5/2011 1:36:53 PM

Sorry, Glen, but I still have an issue with the amount of FAF Micro Algae Grow. 1 ml = 1 cc. They are equivalent. Are you really using 10 ml (or 10 cc) of FAF Micro Algae Grow, undiluted, per 2 L bottle, per day?

rgrking
9/5/11
1:40:14 PM

no I'm sorry. No excuse. It's 1/10th of a mL. I'm using a 1mL syringe to do the dosing.

JimWelsh
9/5/11
1:44:3 PM

OK fix the report and the journal, please, and I think we're good to go!

JimWelsh
9/6/11
11:5:26 PM

Those pesky decimal points are getting in the way! The reports says 0.1 ml, but the journal says 0.01 ml. Please fix the journal.

rgrking
9/7/11
5:42:14 PM

ah I hope I got it lol. Journal matches, sir.

fishtal
10/1/2011 6:31:37 PM

Sorry for the delay. Did you change your salinity?

rgrking
10/1/11
10:33:27 PM

no still staying the same. right at 1.022. I did start adding .5mL daily of the F/2. The cultures are doing great.

fishtal
10/14/2011 9:18:51 PM

Sorry Glen, your journal says 1.024.

rgrking
10/14/11
10:5:17 PM

I fixed it. I also looked and think everything else is ok. Thanks again!

rgrking
10/15/2011 9:42:32 PM

is there something here I'm missing?

fishtal
10/15/2011 9:47:50 PM

Nope, you're good now.

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